MicroRNA and mRNA expression associated with ectopic germinal centers in thymus of myasthenia gravis.

ABSTRACT:

Background

A characteristic pathology of early onset myasthenia gravis is thymic hyperplasia with ectopic germinal centers (GC). However, the mechanisms that trigger and maintain thymic hyperplasia are poorly characterized. Dysregulation of small, non-coding microRNAs (miRNAs) and their target genes has been identified in the pathology of several autoimmune diseases. We assessed the miRNA and mRNA profiles of the MG thymus and have investigated their role in GC formation and maintenance.

Methods

MG thymus samples were assessed by histology and grouped based upon the appearance of GC; GC positive and GC negative. A systems biology approach was used to study the differences between the groups. Our study included miRNA and mRNA profiling, quantitative real-time PCR validation,

SUBMITTER: Sengupta M 

PROVIDER: S-EPMC6181382 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature