Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection.

ABSTRACT:

Background

In order to understand the role of dengue virus (DENV) specific T cell responses that associate with protection, we studied their frequency and phenotype in relation to clinical disease severity and resolution of viraemia in a large cohort of patients with varying severity of acute dengue infection.

Methodology/principal findings

Using ex vivo IFNγ ELISpot assays we determined the frequency of dengue viral peptide (DENV)-NS3, NS1 and NS5 responsive T cells in 74 adult patients with acute dengue infection and examined the association of responsive T cell frequency with the extent of viraemia and clinical disease severity. We found that total DENV-specific and DENV-NS3-specific T cell responses, were higher in patients with dengue fever (DF), when compared to those

SUBMITTER: Wijeratne DT 

PROVIDER: S-EPMC6181435 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature