L-plastin phosphorylation regulates the early phase of sealing ring formation by actin bundling process in mouse osteoclasts.

ABSTRACT: The process of sealing ring formation requires major actin filament reorganization. We previously demonstrated that an actin-bundling protein L-plastin has a role in the cross-linking of actin filaments into tight bundles and forms actin aggregates (denoted as nascent sealing zones). These nascent sealing zones mature into fully functional sealing rings. We have shown here that TNF-alpha signaling regulates the phosphorylation of serine-5 and -7 in L-plastin which increases the actin bundling capacity of L-plastin and hence the formation of nascent sealing zones in mouse osteoclasts. Using the TAT-mediated transduction method, we confirmed the role of L-plastin in nascent sealing zones formation at the early phase of the sealing ring assembly. Transduction of TAT-fused full-length L-plasti

SUBMITTER: Chellaiah MA 

PROVIDER: S-EPMC6181588 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature