Loss of specificity variants of WzxC suggest that substrate recognition is coupled with transporter opening in MOP-family flippases.

ABSTRACT: Bacteria produce a variety of surface-exposed polysaccharides important for cell integrity, biofilm formation and evasion of the host immune response. Synthesis of these polymers often involves the assembly of monomer oligosaccharide units on the lipid carrier undecaprenyl-phosphate at the inner face of the cytoplasmic membrane. For many polymers, including cell wall peptidoglycan, the lipid-linked precursors must be transported across the membrane by flippases to facilitate polymerization at the membrane surface. Flippase activity for this class of polysaccharides is most often attributed to MOP (Multidrug/Oligosaccharidyl-lipid/Polysaccharide) family proteins. Little is known about how this ubiquitous class of transporters identifies and translocates its cognate precursor over the many d

SUBMITTER: Sham LT 

PROVIDER: S-EPMC6181778 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature