Antibiotic export by efflux pumps affects growth of neighboring bacteria.

ABSTRACT: Cell-cell interactions play an important role in bacterial antibiotic resistance. Here, we asked whether neighbor proximity is sufficient to generate single-cell variation in antibiotic resistance due to local differences in antibiotic concentrations. To test this, we focused on multidrug efflux pumps because recent studies have revealed that expression of pumps is heterogeneous across populations. Efflux pumps can export antibiotics, leading to elevated resistance relative to cells with low or no pump expression. In this study, we co-cultured cells with and without AcrAB-TolC pump expression and used single-cell time-lapse microscopy to quantify growth rate as a function of a cell's neighbors. In inhibitory concentrations of chloramphenicol, we found that cells lacking functional efflux p

SUBMITTER: Wen X 

PROVIDER: S-EPMC6181935 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature