Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model.

ABSTRACT: The development of in vitro 3D models to get insights into the mechanisms of bone regeneration could accelerate the translation of experimental findings to the clinic, reducing costs and duration of experiments. This work explores the design and manufacturing of multi-compartments structures in poly(ε-caprolactone) (PCL) 3D-printed by Fused Filament Fabrication technique. The construct was designed with interconnected stalls to host stem cells and endothelial cells. Cells were encapsulated within an optimised gellan gum (GG)-based hydrogel matrix, crosslinked using strontium (Sr²⁺) ions to exploit its bioactivity and finally, assembled within compartments with different sizes. Calcium (Ca²⁺)-crosslinked gels were also used as control for comparison of Sr²⁺

SUBMITTER: De Giglio E 

PROVIDER: S-EPMC6181937 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature