Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria.

ABSTRACT: Conventional implementations of two-dimensional electronic spectroscopy typically spatially average over ~10¹⁰ chromophores spread over ~10⁴ micron square area, limiting their ability to characterize spatially heterogeneous samples. Here we present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially varying differences in excitonic structure, with sensitivity orders of magnitude better than conventional spatially-averaged electronic spectroscopies. The approach performs fluorescence-detection-based fully collinear two-dimensional electronic spectroscopy in a microscope, combining femtosecond time-resolution, sub-micron spatial resolution, and the sensitivity of fluorescence detection. We demonstrate the approach on a mixture of

SUBMITTER: Tiwari V 

PROVIDER: S-EPMC6181999 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature