CGT-seq: epigenome-guided de novo assembly of the core genome for divergent populations with large genome.

ABSTRACT: Genetic diversity in plants is remarkably high. Recent whole genome sequencing (WGS) of 67 rice accessions recovered 10,872 novel genes. Comparison of the genetic architecture among divergent populations or between crops and wild relatives is essential for obtaining functional components determining crucial traits. However, many major crops have gigabase-scale genomes, which are not well-suited to WGS. Existing cost-effective sequencing approaches including re-sequencing, exome-sequencing and restriction enzyme-based methods all have difficulty in obtaining long novel genomic sequences from highly divergent population with large genome size. The present study presented a reference-independent core genome targeted sequencing approach, CGT-seq, which employed epigenomic information from both

SUBMITTER: Qi M 

PROVIDER: S-EPMC6182137 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature