The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.

ABSTRACT: The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD⁺, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for energy conservation and autotrophic growth. We describe here a methodology for markerless mutagenesis in the model bacterium of this class, Acetobacterium woodii, which enabled us to delete the rnf genes and to test their in vivo role. The rnf mutant did not grow on H₂ plus CO₂, nor did it produce acetate or ATP from H₂ plus CO₂, and ferredoxin:NAD⁺ oxidoreductase activity and Na⁺

SUBMITTER: Westphal L 

PROVIDER: S-EPMC6182241 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature