Development of fluorescence quenching in Chlamydomonas reinhardtii upon prolonged illumination at 77 K.

ABSTRACT: Low-temperature fluorescence measurements are frequently used in photosynthesis research to assess photosynthetic processes. Upon illumination of photosystem II (PSII) frozen to 77 K, fluorescence quenching is observed. In this work, we studied the light-induced quenching in intact cells of Chlamydomonas reinhardtii at 77 K using time-resolved fluorescence spectroscopy with a streak camera setup. In agreement with previous studies, global analysis of the data shows that prolonged illumination of the sample affects the nanosecond decay component of the PSII emission. Using target analysis, we resolved the quenching on the PSII-684 compartment which describes bulk chlorophyll molecules of the PSII core antenna. Further, we quantified the quenching rate constant and observed that as the illum

SUBMITTER: Wlodarczyk LM 

PROVIDER: S-EPMC6182390 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature