Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum.

ABSTRACT:

Background

The canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, including cytotoxic chaetoglobosin A (ChA), as well as abundant cellulase and xylanase.

Results

Here we report on the functional characterization of this signaling pathway in C. globosum. We blocked the pathway by knocking down the putative Gα-encoding gene gna1 (in the pG14 mutant). This led to impaired cellulase production and significantly decreased transcription of the major cellulase and xylanase genes. Almost all the glycohydrolase family genes involved in cellulose degradation were downregulated, including the major cellulase genes, cel7a, cel6a, egl1

SUBMITTER: Hu Y 

PROVIDER: S-EPMC6182798 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature