DNA methylation patterns in peripheral blood mononuclear cells from Holstein cattle with variable milk yield.

ABSTRACT:

Background

Milk yield for Holstein cows has doubled over five decades due to genetic selection and changes to management, but the molecular mechanisms that facilitated this increase are mostly unknown. Epigenetic modifications to the cattle genome are a plausible molecular mechanism to cause variation in milk yield and our objective was to describe genome-wide DNA methylation patterns in peripheral blood mononuclear cells (PBMC) from mature Holstein dairy cows with variable milk yield.

Results

Whole genome MeDIP-seq was performed following DNA extraction from PBMC of 6 lactating dairy cows from 4 different herds that varied in milk yield from 13,556 kg to 23,105 kg per 305 day lactation. We describe methylation across the genome and for 13,677 protein coding genes. Repetitiv

SUBMITTER: Dechow CD 

PROVIDER: S-EPMC6182825 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature