Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR.

ABSTRACT:

Background

Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this species. Real-time quantitative PCR (qPCR) has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. In the present study, nine candidate reference genes were rigorously tested for their application in normalization of qPCR data onto roots of four distinct cowpea accessions under two abiotic stresses: root dehydration and salt (NaCl, 100 mM). In addition, the regulation of four target transcripts, under the same referred conditions was also scrutinized.

Results

geNorm, NormFinder, BestKeeper, and ΔCt meth

SUBMITTER: Amorim LLB 

PROVIDER: S-EPMC6182843 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature