Modified SureSelect^QXT Target Enrichment Protocol for Illumina Multiplexed Sequencing of FFPE Samples.

ABSTRACT:

Background

Personalised medicine is nowadays a major objective in oncology. Molecular characterization of tumours through NGS offers the possibility to find possible therapeutic targets in a time- and cost-effective way. However, the low quality and complexity of FFPE DNA samples bring a series of disadvantages for massive parallel sequencing techniques compared to high-quality DNA samples (from blood cells, cell cultures, etc.).

Results

We performed several experiments to understand the behaviour of FFPE DNA samples during the construction of SureSelect^QXT libraries. First, we designed a quality checkpoint for FFPE DNA samples based on the quantification of their amplification capability (qcPCR). We observed that FFPE DNA samples can be classified according to DI

SUBMITTER: Rosa-Rosa JM 

PROVIDER: S-EPMC6182866 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature