Mesophilic Sporeformers Identified in Whey Powder by Using Shotgun Metagenomic Sequencing.

ABSTRACT: Spoilage and pathogenic spore-forming bacteria are a major cause of concern for producers of dairy products. Traditional agar-based detection methods employed by the dairy industry have limitations with respect to their sensitivity and specificity. The aim of this study was to identify low-abundance sporeformers in samples of a powdered dairy product, whey powder, produced monthly over 1 year, using novel culture-independent shotgun metagenomics-based approaches. Although mesophilic sporeformers were the main target of this study, in one instance thermophilic sporeformers were also targeted using this culture-independent approach. For comparative purposes, mesophilic and thermophilic sporeformers were also tested for within the same sample using culture-based approaches. Ultimately, the ap

SUBMITTER: McHugh AJ 

PROVIDER: S-EPMC6182908 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature