An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells.

ABSTRACT: Background: Mast cells are tissue-resident inflammatory cells defined by their high granularity and surface expression of the high-affinity IgE receptor, FcεRI, and CD117/KIT, the receptor for stem cell factor (SCF). There is a considerable heterogeneity among mast cells, both phenotypically and functionally. Human mast cells are generally divided into two main subtypes based on their protease content; the mucosa-associated MC_T (tryptase positive and chymase negative mast cell) and the connective tissue associated-residing MC_TC (tryptase and chymase positive mast cell). Human lung mast cells exhibit heterogeneity in terms of cellular size, expression of cell surface receptors, and secreted mediators. However, knowledge about human lung mast cell heterogeneity i

SUBMITTER: Ravindran A 

PROVIDER: S-EPMC6183502 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature