Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.

ABSTRACT:

Background

Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable. The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice. However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown.

Methods

We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models. We quantified colonic Casp11 mRNA using quantative polymerase chain reaction and colitis using weight loss, blinded histological scoring, IL-12/23p40 secretion by colonic explants, and fecal lipocalin-2. We determined fecal microbial composition usi

SUBMITTER: Fan TJ 

PROVIDER: S-EPMC6185382 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature