Dynamic microRNA activity identifies therapeutic targets in trastuzumab-resistant HER2⁺ breast cancer.

ABSTRACT: MicroRNAs (miRNAs) are implicated in numerous physiologic and pathologic processes, such as the development of resistance to chemotherapy. Determining the role of miRNAs in these processes is often accomplished through measuring miRNA abundance by polymerase chain reaction, sequencing, or microarrays. We have developed a system for the large-scale monitoring of dynamic miRNA activity and have applied this system to identify the contribution miRNA activity to the development of trastuzumab resistance in a cell model of HER2⁺ breast cancer. MiRNA activity measurements identified significantly different activity levels between BT474 cells (HER2 ⁺ breast cancer) and BT474R cells (HER2 ⁺ breast cancer cells selected for resistance to trastuzumab). We created a l

SUBMITTER: Decker JT 

PROVIDER: S-EPMC6185764 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature