Only a subpopulation of mouse sperm displays a rapid increase in intracellular calcium during capacitation.

ABSTRACT: Mammalian sperm must undergo a functionally defined process called capacitation to be able to fertilize oocytes. They become capacitated in vivo by interacting with the female reproductive tract or in vitro in a defined capacitation medium that contains bovine serum albumin, calcium (Ca²⁺ ), and bicarbonate (HCO₃^- ). In this work, sperm were double stained with propidium iodide and the Ca²⁺ dye Fluo-4 AM and analyzed by flow cytometry to determine changes in intracellular Ca²⁺ concentration ([Ca²⁺ ]_i ) in individual live sperm. An increase in [Ca²⁺ ]_i was observed in a subpopulation of capacitated live sperm when compared with noncapacitated ones. Sperm exposed to the capacitating medium disp

SUBMITTER: Luque GM 

PROVIDER: S-EPMC6185779 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature