Photosensitivity and type I IFN responses in cutaneous lupus are driven by epidermal-derived interferon kappa.

ABSTRACT:

Objective

Skin inflammation and photosensitivity are common in patients with cutaneous lupus erythematosus (CLE) and systemic lupus erythematosus (SLE), yet little is known about the mechanisms that regulate these traits. Here we investigate the role of interferon kappa (IFN-κ) in regulation of type I interferon (IFN) and photosensitive responses and examine its dysregulation in lupus skin.

Methods

mRNA expression of type I IFN genes was analysed from microarray data of CLE lesions and healthy control skin. Similar expression in cultured primary keratinocytes, fibroblasts and endothelial cells was analysed via RNA-seq. IFNK knock-out (KO) keratinocytes were generated using CRISPR/Cas9. Keratinocytes stably overexpressing IFN-κ were created via G418 selection of transf

SUBMITTER: Sarkar MK 

PROVIDER: S-EPMC6185784 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature