Characterization of mucoid and serous middle ear effusions from patients with chronic otitis media: implication of different biological mechanisms?

ABSTRACT:

Background

Chronic otitis media with effusion (COME) is characterized by persistent middle ear effusions that are in most cases highly viscous, but some patients present with serous fluid. This study aimed at comprehensively characterizing the macromolecular composition of mucoid vs. serous middle ear effusions (MEEs).

Methods

MEEs from patients with COME were analyzed for proteins by mass spectrometry (MS) and western blot techniques, total DNA quantity, bacterial DNA (16S sequencing), and cytokine content. Proteomics datasets were studied in Ingenuity Pathway Analysis (IPA).

Results

Mucoid samples showed a global tendency of increased pro-inflammatory mediators. Interleukin-1β (IL-1β) and IL-10 were significantly more abundant in serous samples (p < 0.01). Mucoid sa

SUBMITTER: Val S 

PROVIDER: S-EPMC6185811 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature