Regulation of Blood Pressure by Targeting Ca_V1.2-Galectin-1 Protein Interaction.

ABSTRACT:

Background

L-type Ca_V1.2 channels play crucial roles in the regulation of blood pressure. Galectin-1 (Gal-1) has been reported to bind to the I-II loop of Ca_V1.2 channels to reduce their current density. However, the mechanistic understanding for the downregulation of Ca_V1.2 channels by Gal-1 and whether Gal-1 plays a direct role in blood pressure regulation remain unclear.

Methods

In vitro experiments involving coimmunoprecipitation, Western blot, patch-clamp recordings, immunohistochemistry, and pressure myography were used to evaluate the molecular mechanisms by which Gal-1 downregulates Ca_V1.2 channel in transfected, human embryonic kidney 293 cells, smooth muscle cells, arteries from Lgasl1^-/- mice, rat, and human p

SUBMITTER: Hu Z 

PROVIDER: S-EPMC6185826 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature