Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells.

ABSTRACT: Astrocytes are important regulators of excitatory synaptic networks. However, astrocytes regulation of inhibitory synaptic systems remains ill defined. This is particularly relevant since GABAergic interneurons regulate the activity of excitatory cells and shape network function. To address this issue, we combined optogenetics and pharmacological approaches, two-photon confocal imaging and whole-cell recordings to specifically activate hippocampal somatostatin or paravalbumin-expressing interneurons (SOM-INs or PV-INs), while monitoring inhibitory synaptic currents in pyramidal cells and Ca²⁺ responses in astrocytes. We found that astrocytes detect SOM-IN synaptic activity via GABA_BR and GAT-3-dependent Ca²⁺ signaling mechanisms, the latter triggering the r

SUBMITTER: Matos M 

PROVIDER: S-EPMC6185912 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature