Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension.

ABSTRACT:

Background

Neem tree serves as a cornucopia for triterpenoids called limonoids that are of profound interest to humans due to their diverse biological activities. However, the biosynthetic pathway that plant employs for the production of limonoids remains unexplored for this wonder tree.

Results

Herein, we report the tracing of limonoid biosynthetic pathway through feeding experiments using ¹³C isotopologues of glucose in neem cell suspension. Growth and development specific limonoid spectrum of neem seedling and time dependent limonoid biosynthetic characteristics of cell lines were established. Further to understand the role of mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways in limonoid biosynthesis, Ultra Performance Liquid Chromatography (UP

SUBMITTER: Aarthy T 

PROVIDER: S-EPMC6186041 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature