Modified genome comparison method: a new approach for identification of specific targets in molecular diagnostic tests using Mycobacterium tuberculosis complex as an example.

ABSTRACT:

Background

The first step of designing any genome-based molecular diagnostic test is to find a specific target sequence. The modified genome comparison method is one of the easiest and most comprehensive ways to achieve this goal. In this study, we aimed to explain this method with the example of Mycobacterium tuberculosis complex and investigate its efficacy in a diagnostic test.

Methods

A specific target was identified using modified genome comparison method and an in-house PCR test was designed. To determine the analytical sensitivity and specificity, 10 standard specimens were used. Also, 230 specimens were used to determine the clinical sensitivity and specificity.

Results

The identity and query cover of our new diagnostic target (5KST) were ≥ 90% with M. tubercu

SUBMITTER: Neshani A 

PROVIDER: S-EPMC6186143 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature