Presynaptic GCaMP expression decreases vesicle release probability at the calyx of Held.

ABSTRACT: Synaptic vesicle (SV) exocytosis is intimately dependent on free local Ca²⁺ near active zones. Genetically encoded calcium indicators (GECIs) have become an indispensable tool to monitor calcium dynamics during physiological responses, and they are widely used as a proxy to monitor activity in neuronal ensembles and at synaptic terminals. However, GECIs' ability to bind Ca²⁺ at physiologically relevant concentration makes them strong candidates to affect calcium homeostasis and alter synaptic transmission by exogenously increasing Ca²⁺ buffering. In the present study, we show that genetically expressed GCaMP6m modulates SV release probability at the mouse calyx of Held synapse. GCaMP6m expression for approximately three weeks decreased initial SV release fo

SUBMITTER: Singh M 

PROVIDER: S-EPMC6186185 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature