Importance of Promyelocytic Leukema Protein (PML) for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.

ABSTRACT: Many DNA virus replication-related proteins are associated with promyelocytic leukemia protein (PML), a component of nuclear domain 10 (ND10), which has been investigated for its potential involvement in viral replication. In the case of Kaposi's sarcoma-associated herpesvirus (KSHV) lytic gene products, K8 (K-bZIP), ORF59, and ORF75 have been shown to colocalize with PML, but its importance in KSHV lytic replication is still unclear. In this study, we analyzed the functional influence of PML on KSHV latency and lytic replication in KSHV-infected primary effusion lymphoma (PEL) cell lines. Stable PML-knockout (BC3-PML^KO) and PML-overexpressing BC3 cells (BC3^PML) were successfully generated and the latency and reactivation status were analyzed. The results demonstrated

SUBMITTER: Hossain MG 

PROVIDER: S-EPMC6186782 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature