Patient and Strain Characteristics Associated With Clostridium difficile Transmission and Adverse Outcomes.

ABSTRACT:

Background

No study has used whole-genome sequencing (WGS) to investigate risk factors for Clostridium difficile (CD) transmission between cases, or assessed the impact of recent acquisition on patient outcome.

Methods

This 20 month retrospective cohort study included consecutive cytotoxin-positive diarrheal samples, which underwent culture, ribotyping, and WGS (Illumina). Sequenced isolates were compared using single nucleotide variants (SNVs). Independent predictors of acquisition from another case, onward transmission, 120-day recurrence, and 30-day mortality were identified using logistic regression with backwards elimination.

Results

Of 660 CD cases, 640 (97%) were sequenced, of which 567 (89%) shared a ribotype with a prior case, but only 227 (35%) were ≤2 SNVs

SUBMITTER: Martin JSH 

PROVIDER: S-EPMC6186849 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature