Maternal immune activation alters brain microRNA expression in mouse offspring.

ABSTRACT:

Objective

Maternal immune activation (MIA) is associated with an increased risk of autism spectrum disorder (ASD) in offspring. Herein, we investigate the altered expression of microRNAs (miRNA), and that of their target genes, in the brains of MIA mouse offspring.

Methods

To generate MIA model mice, pregnant mice were injected with polyriboinosinic:polyribocytidylic acid on embryonic day 12.5. We performed miRNA microarray and mRNA sequencing in order to determine the differential expression of miRNA and mRNA between MIA mice and controls, at 3 weeks of age. We further identified predicted target genes of dysregulated miRNAs, and miRNA-target interactions, based on the inverse correlation of their expression levels.

Results

Mice prenatally subjected to MIA exhibited

SUBMITTER: Sunwoo JS 

PROVIDER: S-EPMC6186947 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature