Confinement Facilitated Protein Stabilization As Investigated by Small-Angle Neutron Scattering.

ABSTRACT: While mesoporous silicas have been shown to be a compelling candidate for drug delivery and the implementation of biotechnological applications requiring protein confinement and immobilization, the understanding of protein behavior upon physical adsorption into silica pores is limited. Many indirect methods are available to assess general adsorbed protein stability, such as Fourier-transform infrared spectroscopy and activity assays. However, the limitation of these methods is that spatial protein arrangement within the pores cannot be assessed. Mesoporous silicas pose a distinct challenge to direct methods, such as transmission electron microscopy, which lacks the contrast and resolution required to adequately observe immobilized protein structure, and nuclear magnetic resonance, which is

SUBMITTER: Siefker J 

PROVIDER: S-EPMC6187370 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature