Multiple levels of gene regulation mediate differentiation of the intracellular pathogen Leishmania.

ABSTRACT: For many years, mRNA abundance has been used as the surrogate measure of gene expression in biological systems. However, recent genome-scale analyses in both bacteria and eukaryotes have revealed that mRNA levels correlate with steady-state protein abundance for only 50-70% of genes, indicating that translation and post-translation processes also play important roles in determining gene expression. What is not yet clear is whether dynamic processes such as cell cycle progression, differentiation, or response to environmental changes change the relationship between mRNA and protein abundance. Here, we describe a systems approach to interrogate promastigote-to-amastigote differentiation in the obligatory intracellular parasitic protozoan Leishmania donovani. Our results indicate that regulat

SUBMITTER: Lahav T 

PROVIDER: S-EPMC6188352 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature