Lens Epithelial Cells Initiate an Inflammatory Response Following Cataract Surgery.

ABSTRACT:

Purpose

Lens epithelial cell (LEC) conversion to myofibroblast is responsible for fibrotic cataract surgery complications including posterior capsular opacification. While transforming growth factor beta (TGFβ) signaling is important, the mechanisms by which the TGFβ pathway is activated post cataract surgery (PCS) are not well understood.

Methods

RNA-seq was performed on LECs obtained from a mouse cataract surgery model at the time of surgery and 24 hours later. Bioinformatic analysis was performed with iPathwayGuide. Expression dynamics were determined by immunofluorescence.

Results

The LEC transcriptome is massively altered by 24 hours PCS. The differentially expressed genes included those important for lens biology, and fibrotic markers. However, the most dramatic

SUBMITTER: Jiang J 

PROVIDER: S-EPMC6188467 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature