Selenium supplementation prevents metabolic and transcriptomic responses to cadmium in mouse lung.

ABSTRACT: Background:The protective effect of selenium (Se) on cadmium (Cd) toxicity is well documented, but underlying mechanisms are unclear.

Methods:Male mice fed standard diet were given Cd (CdCl₂, 18 μmol/L) in drinking water with or without Se (Na₂SeO_4, 20 μmol/L) for 16 weeks. Lungs were analyzed for Cd concentration, transcriptomics and metabolomics. Data were analyzed with biostatistics, bioinformatics, pathway enrichment analysis, and combined transcriptome-metabolome-wide association study.

Results:Mice treated with Cd had higher lung Cd content (1.7 ± 0.4 pmol/mg protein) than control mice (0.8 ± 0.3 pmol/mg protein) or mice treated with Cd and Se (0.4 ± 0.1 pmol/mg protein). Gene set enrichment analysis of transcriptomi

SUBMITTER: Hu X 

PROVIDER: S-EPMC6188826 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature