Raman micro-spectroscopy monitors acquired resistance to targeted cancer therapy at the cellular level.

ABSTRACT: Monitoring the drug efficacy or resistance in vitro is usually carried out by measuring the response of single few proteins. However, observation of single proteins instead of an integral cell response may lead to results that are not consistent with patient's response to a drug. We present a Raman spectroscopic method that detects the integral cell response to drugs such as tyrosine kinase inhibitors (TKIs). Non-small cell lung cancer (NSCLC) patients with EGFR mutations develop acquired resistance to first (erlotinib)- and third (osimertinib)-generation TKIs. Large erlotinib-induced differences were detected by Raman micro-spectroscopy in NSCLC cells without T790M EGFR mutation but not in cells with this mutation. Additionally, Raman difference spectra detected the response of NSCLC cell

SUBMITTER: Hammoud MK 

PROVIDER: S-EPMC6189084 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature