Fine-tuning carbapenem resistance by reducing porin permeability of bacteria activated in the selection process of conjugation.

ABSTRACT: Antibiotic resistance is an emerging public health issue. Plasmids are one of the popular carriers to disseminate resistance genes among pathogens. However, the response of plasmid-carrying bacteria to antibiotic treatment and how these bacteria evolve to increase their resistance remain elusive. In this study, we conjugated plasmid pNDM-HK to E. coli J53 recipient cells and selected survivors using different concentrations of the broad spectrum antibiotic meropenem. After selection, transconjugants conferred varying minimum inhibitory concentrations with respect to carbapenems. We sequenced and compared the transcriptomes of transconjugants that exhibited distinct carbapenem susceptibilities, and found that the loss of outer membrane proteins led to antibiotic resistance. Moreover, we ide

SUBMITTER: Kong HK 

PROVIDER: S-EPMC6189183 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature