Use of flow cytometry and total viable count to determine the effects of orange juice composition on the physiology of Escherichia coli.

ABSTRACT: Orange juice (OJ) contains numerous compounds some of which are known to play key roles in growth and survival of bacteria. This study aimed to investigate the effects of natural or processing-induced variations in OJ composition on the physiology of Escherichia coli. OJ and model OJ (MOJ) samples containing various sugars, organic acids, amino acids, or ascorbic acid were inoculated with E. coli K-12 MG1655 in different growth phases. The culturability, viability, and physiology of the cells were investigated during storage using plate counting and flow cytometry. Generally, stationary-phase cells displayed the greatest survival in both MOJ and OJ. Increase in incubation temperature from 4 to 22.5ºC caused a significant decrease in both healthy and culturable cell population

SUBMITTER: Anvarian AHP 

PROVIDER: S-EPMC6189610 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature