Choline sulfatase from Ensifer (Sinorhizobium) meliloti: Characterization of the unmodified enzyme.

ABSTRACT: Ensifer (Sinorhizobium) meliloti is a nitrogen-fixing α-proteobacterium able to biosynthesize the osmoprotectant glycine betaine from choline sulfate through a metabolic pathway that starts with the enzyme choline-O-sulfatase. This protein seems to be widely distributed in microorganisms and thought to play an important role in their sulfur metabolism. However, only crude extracts with choline sulfatase activity have been studied. In this work, Ensifer (Sinorhizobium) meliloti choline-O-sulfatase was obtained in a high degree of purity after expression in Escherichia coli. Gel filtration and dynamic light scattering experiments showed that the recombinant enzyme exists as a dimer in solution. Using calorimetry, its catalytic ac

SUBMITTER: Sanchez-Romero JJ 

PROVIDER: S-EPMC6189696 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature