Baking Powder Actuated Centrifugo-Pneumatic Valving for Automation of Multi-Step Bioassays.

ABSTRACT: We report a new flow control method for centrifugal microfluidic systems; CO₂ is released from on-board stored baking powder upon contact with an ancillary liquid. The elevated pressure generated drives the sample into a dead-end pneumatic chamber sealed by a dissolvable film (DF). This liquid incursion wets and dissolves the DF, thus opening the valve. The activation pressure of the DF valve can be tuned by the geometry of the channel upstream of the DF membrane. Through pneumatic coupling with properly dimensioned disc architecture, we established serial cascading of valves, even at a constant spin rate. Similarly, we demonstrate sequential actuation of valves by dividing the disc into a number of distinct pneumatic chambers (separated by DF membranes). Opening these DFs, typically throu

SUBMITTER: Kinahan DJ 

PROVIDER: S-EPMC6189914 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature