Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices.

ABSTRACT: FTA® paper can be used to protect a variety of biological samples prior to analysis, facilitating ease-of-transport to laboratories or long-term archive storage. The use of FTA® paper as a solid phase eradicates the need to elute the nucleic acids from the matrix prior to DNA amplification, enabling both DNA purification and polymerase chain reaction (PCR)-based DNA amplification to be performed in a single chamber on the microfluidic device. A disc of FTA® paper, containing a biological sample, was placed within the microfluidic device on top of wax-encapsulated DNA amplification reagents. The disc containing the biological sample was then cleaned up using Tris-EDTA (TE) buffer, which was passed over the disc, via electro-osmotic flow, in order to remove any potential inhibitors of downst

SUBMITTER: Wimbles R 

PROVIDER: S-EPMC6190317 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature