A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue.

ABSTRACT: Due to the abuse of antibiotics, antibiotic residues can be detected in both natural environment and various industrial products, posing threat to the environment and human health. Here we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline (Tc)-one of the commonly used antibiotics once on humans and now on poultry, cattle and fisheries. A Tc-degrading enzyme, TetX, from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in E. coli and fully characterized, including its K _m and k _cat value. We quantitatively evaluated its activity both in vitro and in vivo by UV-Vis spectrometer and LC-MS. Moreover, we used a tetracycline inducible amp

SUBMITTER: Mu Z 

PROVIDER: S-EPMC6190513 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature