Method for hydrophytic plant sample preparation for light and electron microscopy (studies on Phragmites australis Cav.).

ABSTRACT: Nowadays there are no well-established, standard methods in electron microscopy despite its 50-year history. An excessive variety of research objects prompt researchers to modify and improve methodological approaches to sample preparation. One of the difficult objects to study by electron microscopy is hydrophytic plants, for example, Phragmites australis Cav. Traditional approaches to fixation and sample preparation do not give satisfactory results due to the peculiarities in structure and physiology of hydrophytic plants. The purpose of present research is modification description of the widespread method developed for double fixation of hydrophytic plant tissue for transmission electron microscopy. Suggested approach takes into account the features of hydrophyte plants. •The deve

SUBMITTER: Fedorenko GM 

PROVIDER: S-EPMC6190527 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature