Determinants of selection in yeast evolved by genome shuffling.

ABSTRACT:

Background

Genome shuffling (GS) is a widely adopted methodology for the evolutionary engineering of desirable traits in industrially relevant microorganisms. We have previously used genome shuffling to generate a strain of Saccharomyces cerevisiae that is tolerant to the growth inhibitors found in a lignocellulosic hydrolysate. In this study, we expand on previous work by performing a population-wide genomic survey of our genome shuffling experiment and dissecting the molecular determinants of the evolved phenotype.

Results

Whole population whole-genome sequencing was used to survey mutations selected during the experiment and extract allele frequency time series. Using growth curve assays on single point mutants and backcrossed derivatives, we explored the genetic a

SUBMITTER: Biot-Pelletier D 

PROVIDER: S-EPMC6190656 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature