Metabolic flux analysis in Ashbya gossypii using ¹³C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions.

ABSTRACT:

Background

The fungus Ashbya gossypii is an important industrial producer of the vitamin riboflavin. Using this microbe, riboflavin is manufactured in a two-stage process based on a rich medium with vegetable oil, yeast extract and different precursors: an initial growth and a subsequent riboflavin production phase. So far, our knowledge on the intracellular metabolic fluxes of the fungus in this complex process is limited, but appears highly relevant to better understand and rationally engineer the underlying metabolism. To quantify intracellular fluxes of growing and riboflavin producing A. gossypii, studies with different ¹³C tracers were embedded into a framework of experimental design, isotopic labeling analysis by MS and NMR techniques, and model-based data process

SUBMITTER: Schwechheimer SK 

PROVIDER: S-EPMC6190667 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature