Simultaneous quantification of straight-chain and branched-chain short chain fatty acids by gas chromatography mass spectrometry.

ABSTRACT: Biomedical research in areas such as metabolic disorders, neuromodulatory, and immunomodulatory conditions involves lipid metabolism and demands a reliable and inexpensive method for quantification of short chain fatty acids (SCFAs). We report a GC-MS method for analysis of all straight-chain and branched-chain SCFAs using pentafluorobenzyl bromide (PFBBr) as derivatization reagent. We optimized the derivatization and GC-MS conditions using a mixture containing all eight SCFA standards, i.e., five straight-chain and three branched-chain SCFAs. The optimal derivatization conditions were derivatization time 90 min, temperature 60 °C, pH 7, and (CH₃)₂CO:H₂O ratio 2:1 (v:v). Comparing the performance of different GC column configurations, a 30 m DB-225ms hyphen

SUBMITTER: He L 

PROVIDER: S-EPMC6190712 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature