Long-Term High-Density Extracellular Recordings Enable Studies of Muscle Cell Physiology.

ABSTRACT: Skeletal (voluntary) muscle is the most abundant tissue in the body, thus making it an important biomedical research subject. Studies of neuromuscular transmission, including disorders of ion channels or receptors in autoimmune or genetic neuromuscular disorders, require high-spatial-resolution measurement techniques and an ability to acquire repeated recordings over time in order to track pharmacological interventions. Preclinical techniques for studying diseases of neuromuscular transmission can be enhanced by physiologic ex vivo models of tissue-tissue and cell-cell interactions. Here, we present a method, which allows tracking the development of primary skeletal muscle cells from myoblasts into mature contracting myotubes over more than 2 months. In contrast to most previous stu

SUBMITTER: Lewandowska MK 

PROVIDER: S-EPMC6190753 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature