Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors.

ABSTRACT: A tissue engineered oesophagus could overcome limitations associated with oesophageal substitution. Combining decellularized scaffolds with patient-derived cells shows promise for regeneration of tissue defects. In this proof-of-principle study, a two-stage approach for generation of a bio-artificial oesophageal graft addresses some major challenges in organ engineering, namely: (i) development of multi-strata tubular structures, (ii) appropriate re-population/maturation of constructs before transplantation, (iii) cryopreservation of bio-engineered organs and (iv) in vivo pre-vascularization. The graft comprises decellularized rat oesophagus homogeneously re-populated with mesoangioblasts and fibroblasts for the muscle layer. The oesophageal muscle reaches organised maturation after dynami

SUBMITTER: Urbani L 

PROVIDER: S-EPMC6191423 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature