Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence.

ABSTRACT:

Background

Anopheles stephensi is considered an important malaria vector in Iran, Asia, and recently in the Horn of Africa. Recently, Ansteobp1 intron I sequence has been introduced a new molecular marker for identification of its biological forms including, mysorensis, intermediate and type, using insectary colony specimens.

Methods

In the current study, new marker ability in molecular identification of biological forms has been evaluated with An. stephensi specimens collected from Iran and Afghanistan malarious provinces. Following DNA extraction and PCR amplification, sequence analysis and constructed phylogenetic tree revealed that type and intermediate forms are distributed in Iran.

Results

The specimens collected from Afghanistan identified as intermediate and m

SUBMITTER: Firooziyan S 

PROVIDER: S-EPMC6191895 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature