Genetic characterization of norovirus GII.4 variants circulating in Canada using a metagenomic technique.

ABSTRACT:

Background

Human norovirus is the leading cause of viral gastroenteritis globally, and the GII.4 has been the most predominant genotype for decades. This genotype has numerous variants that have caused repeated epidemics worldwide. However, the molecular evolutionary signatures among the GII.4 variants have not been elucidated throughout the viral genome.

Method

A metagenomic, next-generation sequencing method, based on Illumina RNA-Seq, was applied to determine norovirus sequences from clinical samples.

Results

Herein, the obtained deep-sequencing data was employed to analyze full-genomic sequences from GII.4 variants prevailing in Canada from 2012 to 2016. Phylogenetic analysis demonstrated that the majority of these sequences belong to New Orleans 2009 and Sydney 2

SUBMITTER: Petronella N 

PROVIDER: S-EPMC6191920 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature