A comparative study of endoderm differentiation in humans and chimpanzees.

ABSTRACT:

Background

There is substantial interest in the evolutionary forces that shaped the regulatory framework in early human development. Progress in this area has been slow because it is difficult to obtain relevant biological samples. Induced pluripotent stem cells (iPSCs) may provide the ability to establish in vitro models of early human and non-human primate developmental stages.

Results

Using matched iPSC panels from humans and chimpanzees, we comparatively characterize gene regulatory changes through a four-day time course differentiation of iPSCs into primary streak, endoderm progenitors, and definitive endoderm. As might be expected, we find that differentiation stage is the major driver of variation in gene expression levels, followed by species. We identify thousands o

SUBMITTER: Blake LE 

PROVIDER: S-EPMC6191992 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature