Quality of biological images, reconstructed using localization microscopy data.

ABSTRACT: Motivation:Fluorescence localization microscopy is extensively used to study the details of spatial architecture of subcellular compartments. This modality relies on determination of spatial positions of fluorophores, labeling an extended biological structure, with precision exceeding the diffraction limit. Several established models describe influence of pixel size, signal-to-noise ratio and optical resolution on the localization precision. The labeling density has been also recognized as important factor affecting reconstruction fidelity of the imaged biological structure. However, quantitative data on combined influence of sampling and localization errors on the fidelity of reconstruction are scarce. It should be noted that processing localization microscopy data is similar to reconstru

SUBMITTER: Ruszczycki B 

PROVIDER: S-EPMC6192211 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature